TY - JOUR T1 - Neuropeptide immunofluorescence in human nasal mucosa: assessment of the technique for vasoactive intestinal peptide (VIP) JO - Brazilian Journal of Otorhinolaryngology (English Edition) T2 - AU - de Mendonça,Jeferson Cedaro AU - Dolci,José Eduardo Lutaif SN - 18088694 M3 - 10.1016/S1808-8694(15)31299-4 DO - 10.1016/S1808-8694(15)31299-4 UR - http://www.bjorl.org/en-neuropeptide-immunofluorescence-in-human-nasal-articulo-S1808869415312994 AB - Neuropeptides are important neurotransmitters in nasal physiology and the increasing knowledge of their role in nasal diseases brings new therapeutic perspectives. The investigation of human nasal mucosa neuropeptides is based mostly on immunocytochemistry, a complex approach whose resulting factors may be variable. Aiming to make this kind of research available, an immunofluorescence approach for vasoactive intestinal peptide (VIP) in human nasal mucosa is proposed and evaluated. Study designTransversal cohort. Material and MethodHuman inferior turbinate samples were obtained at time of nasal surgery from eight patients. The samples were fixed in Zamboni solution (4% phosphate-buffered paraformaldehyde and 0.4% picric acid), snap-frozen and stored at -70ºC. 14 µm sections were then obtained. Immunofluorescence staining for VIP (Peninsula Laboratories) was performed and its images documented by conventional photography. The method's specificity, sensitivity and reproducibility of execution were evaluated. Additionally, the reproducibility of interpretation of results was evaluated through the comparison of staining scores (0 to 4) attributed to the images by six observers. ResultsThe results showed the approach to be very specific and sensible, besides being reproducible in its execution. The interpretation of results may depend on the observer's accuracy in judging immunofluorescence images, but it showed uniformity. ConclusionThe proposed method was highly useful for research purposes in neuropeptides in human nasal mucosa. ER -